Custom DNA Synthesis

• Unmodified primers
• Proprietary methodology in making poly “G” sequences and their purifications
• Modified bases, 3', 5', or internally modified oligonucleotides
• Dual Dye labeled DNA synthesis such as Scorpion oligonucleotides
• Other fluorescent labeled synthesis including Tamra, Fluorescein, FAM, etc.
• Phosphorothioate modified oligonucleotides
• 3'-and 5'-disulfide, biotinylated, spacer, apurinic, 5'-and 3' labeled phosphate
• Multi-step organic synthesis and incorporating these modified nucleoside derivatives into making custom DNA
  
  
  Codon 60-62 of the human N-ras proto-oncogene containing benzo[a]pyrene diol epoxide adduct at the dA residue

Other Custom DNA synthesis includes:

• Back bone modifications such as phosphoramidate, phosphorothioate, ethylene glycol and morpholino
  backbone to be used as antisense oligonucleotides.
• PNA-DNA conjugates

Surface modification:

• Attachment of amino and other custom DNA to Luminex beads.
• Modification of gold surface using thio modified oligonucleotides.
• Attachment of custom primers to glass surface.

Long mer Oligos

• Synthesis of oligomers up to 150-160 base pairs.
• We provide mass spectra to confirm the final product.
• We have a proprietary method of purification for these types of oligos.

Custom RNA synthesis:

• Modified and labeled RNA synthesis

Quality Control of custom DNA and Primers (HPLC, Mass Spectral analysis and Capillary Electrophoresis):

PrimeSyn Lab Inc. has proprietary HPLC purification methodology whereby it is possible to achieve very difficult separation in case of modified oligonucleotides and sequences containing multiple "G" and "C" bases. Our final product undergoes stringent testing before shipping. We have ion-exchange and reverse-phase methodologies which enables us to even separate sequences containing wobble bases. PrimeSyn Lab also uses Capillary Electrophoresis (P/ACE™ MDQ ) , which is an excellent analytical technique with high sensitivity, high resolution, and has a robust ability for determining the purity of the DNA.

Each custom DNA synthesized by PSL undergoes stringent quality control such as HPLC, and mass spectral analysis. PSL offers complete guarantee for synthesized unmodified primers or other custom DNA to exceed the customer’s satisfaction. We stand behind our product and customers can ask for product replacement within 30 days if they are not completely satisfied with the custom primers or any other products. A comprehensive certificate of analysis provided with each custom DNA or primers includes information such as: sequence, purchase order number, lot number, concentration, and the total number of OD. PSL can make custom label based on their clients needs. PSL Confirms molecular mass ( +/- 5 amu) for synthetic oligomers up to 100 mer which helps us to monitor effieciency of synthesis and capping of each custom DNA primers. The importance of mass spectroscopy in the quality control of custom DNA and primers are proven by the following example. In one such synthesis, we observed multiple +28 peak due to the N-formyl group, a result of incomplete cleavage of dimethylformamidine group on "G" bases in the presence of ammonium hydroxide at room temperature. Interestingly, both HPLC and capillary electrophoresis indicated one peak. Re-suspension in 30% ammonium hydroxide at 55 oC for two hours resulted in complete disappearance of the +28 amu peak.

For Custom DNA order or quote:

Please download the form and send us to us via either an email or fax.